A differential nucleic acid fluorescent stain applied to cell culture systems

Methyl-green-pyronin as a differential nucleic acid stain for peripheral blood smears.

Fluorescent nucleic acid base analogues.

The use of fluorescent nucleic acid base analogues is becoming increasingly important in the fields of biology, biochemistry and biophysical chemistry as well as in the field of DNA nanotechnology. The advantage of being able to incorporate a fluorescent probe molecule close to the site of examination in the nucleic acid-containing system of interest with merely a minimal perturbation to the na...

Fluorescent protein biosensors applied to microphysiological systems.

This mini-review discusses the evolution of fluorescence as a tool to study living cells and tissues in vitro and the present role of fluorescent protein biosensors (FPBs) in microphysiological systems (MPSs). FPBs allow the measurement of temporal and spatial dynamics of targeted cellular events involved in normal and perturbed cellular assay systems and MPSs in real time. FPBs evolved from fl...

A Novel Fuzzy-Genetic Differential Evolutionary Algorithm for Optimization of A Fuzzy Expert Systems Applied to Heart Disease Prediction

This study presents a novel intelligent Fuzzy Genetic Differential Evolutionary model for the optimization of a fuzzy expert system applied to heart disease prediction in order to reduce the risk of heart disease. To this end, a fuzzy expert system has been proposed for the prediction of heart disease. The proposed model can be used as a tool to assist physicians. In order to: (1) tune the para...

Nucleic acid fluorescent probes for biological sensing.

Nucleic acid fluorescent probes are playing increasingly important roles in biological sensing in recent years. In addition to the conventional functions of single-stranded DNA/RNA to hybridize with their complementary strands, affinity nucleic acids (aptamers) with specific target binding properties have also been developed, which has greatly broadened the application of nucleic acid fluoresc...